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Carrier proteins and coupling methods
For peptide-based immunisation procedures, the use of peptide-carrier protein conjugates is recommended other than in studies where the MAP approach described earlier is being used.
Keyhole limpet haemocyanin (KLH) is routinely used as a carrier protein, with conjugation of the peptide to the carrier protein being effected via an N-terminal cysteine or C-terminal cysteine amide residue (natural or added), using an MBS-mediated optimised coupling procedure.
Other carrier proteins (e.g. BSA, ovalbumin, thyroglobulin, tetanus toxoid, diphtheria toxoid, tuberculin PPD) and conjugation procedures (e.g. glutaraldehyde, bis-diazotised tolidine (BDT), carbodiimide) are available upon request.
In addition to its peptide conjugation expertise, AFFINITI has considerable experience of the preparation of matrices for immunoisolation and in the immobilisation of enzymes for continuous flow applications where retention of a protein’s chemical and physical integrity is essential for subseqent activity. Please contact AFFINITI Customer Service for additional information and assistance.
